منابع مشابه
Multilocus sequence typing of Clostridium difficile.
A robust high-throughput multilocus sequence typing (MLST) scheme for Clostridium difficile was developed and validated using a diverse collection of 50 reference isolates representing 45 different PCR ribotypes and 102 isolates from recent clinical samples. A total of 49 PCR ribotypes were represented overall. All isolates were typed by MLST and yielded 40 sequence types (STs). A web-accessibl...
متن کاملBacteriophage and bacteriocin typing scheme for Clostridium difficile.
The study of the epidemiology of infection with Clostridium difficile would be aided by a way to type individual bacterial isolates. We therefore sought bacteriophages for use in typing. With mitomycin C exposure (3 micrograms/ml), filtrates from 10 strains of C. difficile had plaque-forming lytic activity on other C. difficile strains. Individual phage were passaged and made into high-titer st...
متن کاملA Preliminary Molecular Typing by PCR Assays of Clostridium perfringens and Clostridium difficile Isolates from Dogs
Clostridium perfringens and C. difficile have been associated with acute and chronic large and small bowel diarrhoea, and acute haemorrhagic diarrhoeal syndrome in dogs. The objective of this study was to investigate by toxin gene profile and PCR-ribotyping the molecular characteristics of 14 C. perfringens and 10 C. difficile isolates from 95 canine faeces (n = 36, diarrhoeic and n = 59, non-d...
متن کاملclostridium difficile
seventy-five meconium samples were examined for the presence of ci. difficile: 3 strains were isola ted. additionally 45 labora tory animal faeces specimens were tested for the same purpose, a further 2 cases were isolated. these five suspicious strains were identified as ci. difficile acco rding to the tests mentioned in the previous paragraphs. the organisms . isolated here showed the same ch...
متن کاملAnalysis of Clostridium difficile
HindIII restriction enzyme digests of genomic DNA from nine distinct strains of Clostridium difficile were undertaken, and the results were related to those of a previously established typing method based on [35S]methionine-labeled protein profiles. Each of the typed strains identified by its protein profile could also be distinguished by its unique DNA digestion pattern. Analysis of strains is...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2001
ISSN: 1198-743X
DOI: 10.1046/j.1198-743x.2001.00288.x